Description
- Formula* per Liter: Pancreatic Digest of Gelatin........... 4.0g Casein Digest Peptone................... 4.0g L-Cystine ...................................... 0.128g Beef Extract .................................. 3.0g Lactose ........................................ 10.0g Bromothymol Blue........................ 0.02g Agar ...............................................15.0g Final pH: 7.3 ± 0.2 at 25.0°C * Grams per liter may be adj. or formula supplemented to obtain desired performance Preparation: Mix 36.1 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes. Quality Control Specifications: 1. The powder is homogeneous, free flowing and light beige. 2. Visually the prepared agar media are clear and light greyish-green in color. 3. Expected cultural response after 18-24 hours at 35.0°C.. Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
Use: CLED Agar is recommended for the cultivation and detection of bacteria in urine. Description: CLED Agar (Cystine-Lactose-Electrolyte-Deficient) media was first formulated by Sandys and later modified by Mackey and Sandys. This medium supports the growth of urinary pathogens and gives good colonial differentiation of these bacteria. Lactose is the carbon source. Bromthymol blue is the indicator dye. bromthymol blue, Organisms that are lactose fermenting produce yellow colonies while non-lactose fermenting bacteria give blue colonies. Since there are no salts (electrolyte-deficient) in this media the swarming of Proteus sp. is suppressed. CLED Agar is recommended as a dip inoculum transport medium for urine specimens. A quantitative count method for urine cultures called the Drop Plate method was devised by Neblett.
